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The aid worker was among 15 humanitarian personnel killed on March 23 in an attack by Israeli forces, according to the United Nations and the Palestinian Red Crescent.
The Israeli military has said its soldiers "did not randomly attack" any ambulances, insisting they fired on "terrorists" approaching them in "suspicious vehicles".
Military spokesman Lieutenant Colonel Nadav Shoshani said that troops opened fire on vehicles that had no prior clearance from Israeli authorities and had their lights off.
But the footage released by the Red Crescent on Saturday appears to contradict the Israeli military's initial claims, showing ambulances travelling with their headlights on and emergency lights flashing.
The six minute 42 second video, apparently filmed from inside a moving vehicle, captures a red firetruck and ambulances driving through the night amid constant automatic gunfire.
The vehicles stop beside another on the roadside, and two uniformed men get out.
In the video, the voices of two medics are heard -- one saying "the vehicle, the vehicle", and another responding: "It seems to be an accident."
Seconds later a volley of gunfire breaks out and the screen goes black.